How long to store infant urine for analysis ~ Children's City Clinic No. 1 of Magnitogorsk

How to store urine for analysis before submitting it to the laboratory

Biological materials are stored exclusively in sterile containers. It is advisable to use purchased plastic containers for biomaterials. It is allowed to take simple glass jars with tight lids and sterilize them.

Urine from infants is often collected using special urinals. The packages are designed taking into account the anatomical characteristics of boys and girls. After collection, it is important to carefully pour into a sterile jar. Some urine bags have a clasp. The finished material is handed over to them immediately.

Suitable container:

special container;
glass jar;
laboratory-issued glassware;
urinal with clasp.

Not suitable:

Plastic container;
plastic bags;
unsealed containers;
metal cans.

After collecting the urine, it is necessary to seal it, put on an additional bag, and place it in a second container. The protection will prevent accidental leakage of liquid and will seal the smell inside.

Urine for analysis is stored at a temperature of +2-4 degrees. Shelf life is from 2 to 24 hours, depending on the type of research. A sterile sealed container must be used.

Urine collection

1. Collecting the mid-morning urine sample

Preparing the Patient to Take a Urine Sample

A morning sample of urine taken immediately after waking up is suitable for analysis.
At least 4-6 hours should pass from the last urination to the time the urine is taken for analysis.
You should not eat or drink 10-14 hours before taking a urine sample. If necessary, you can drink one glass of pure still water without any additives.
Before taking a sample you should avoid: Physical and emotional stress
Taking medications

It is important to know

The first morning urine sample is the standard material for dipstick testing, sediment microscopy, urinary albumin-to-creatinine ratio determination, and microbiological tests.
Urine should be collected immediately into a container intended for this purpose. Pouring from one container to another is not permitted.
The container for collecting urine samples must be sterile and have a tight-fitting lid.
Do not touch the inside of the cookware or place foreign objects inside.
All accessories for sample collection (cups, test tubes, tubes) are intended for one-time use.
You can ask the laboratory or doctor for the necessary supplies. It is allowed to use only this cookware; the use of any other cookware (household) is not permitted.
Within 15 minutes after collecting urine, you need to transfer the urine from the cup to vacuum tubes.
For infants and young children, urine collection bags should be used. After thoroughly toileting the child’s external genitalia, you should secure the bag and collect the excreted urine for 10-15 minutes. Using a special tube, transfer urine from the bag to a test tube.

Urine collection

Before urinating, the patient should wash the external genitalia with warm running water. Detergents (soap, washcloth, etc.) cannot be used. It is important to wash the external opening of the urethra. To do this, in women you need to spread the labia with your fingers, in men you need to move the foreskin back.
Gently dry the external genitalia with a paper towel.
If women have menstrual or any other discharge at the time of urine collection for analysis, then it is necessary to use a vaginal tampon.
When urinating, make sure that the external opening of the urethra is exposed.
To collect urine, remove the lid from the cup and place it next to it with the sticker down. NB! Do not peel off the sticker from the cup lid!
To collect urine, a few milliliters of urine should be flushed down the toilet, then, without interrupting the flow, it should be directed into a container, filling the glass three-quarters full. You can then finish urinating into the toilet.
After collecting urine, close the container tightly with a lid.
Within 15 minutes after collecting the urine, transfer the urine from the cup to the test tubes. NB! Do not remove the caps from the test tubes!
If you need to fill several test tubes, first fill the test tube with the green stopper, then the test tube with the beige stopper.

To transfer urine into test tubes:

1. Using a special cup with a needle in the lid:

Partially remove the sticker from the lid (do not throw it away). In the hole you will see a latex-covered needle.
Insert the test tube into the hole in the glass with the lid facing forward and press until it stops (the needle will pierce the stopper).
Hold the test tube until the stream of urine stops flowing into the test tube.
Remove the test tube from the hole and, if necessary, fill the next one in the same way.

2. Using a sterile cup and a special tube (performed by a medical professional due to the risk of injury):

Remove the cap from the sample cup.
Point the thin end of the tube into a container with urine.
Insert the test tube (cap first) into the wide opening of the tube (the needle will pierce the stopper).
Hold the test tube until the stream of urine stops flowing into the test tube.
Remove the tube from the hole and, if necessary, fill the next one.

Turn the test tube upside down and back again with smooth movements 8-10 times so that the addition in the test tube dissolves.
Label the tube(s): write a name on the side of the tube or stick a barcode vertically from top to bottom, as straight and smooth as possible. Also write the date and time of urine collection.
Sample Collection Supplies Used: Make sure the opening on the cup lid is sealed tightly with adhesive tape.
Pour the remaining urine in the cup into the toilet.
Dispose of the urine cup in your household waste container
Specimens should be taken to the laboratory/doctor on the same day the urine was collected.
Store samples at +2...+8°C until you take them to the laboratory or doctor.

2. Urine collection (catheterization)

Before puncture of a permanent catheter, it should be closed for 0.5-1 hour.
The surface of the catheter is cleaned with a disinfectant solution.
The catheter is punctured with a sterile cannula or needle.
Urine is collected in a special container.
NB! Material from a urinal bag is not suitable for research.
The container must be marked.

3. Urine collection for nephrostomy

The surface around the stoma and the stoma itself are thoroughly cleaned with a disinfectant solution.
Place a new bag to collect urine.
Using a sterile pipette, transfer the required amount of urine from the urine collection bag to a sterile sample container.
Transfer urine from a sterile container to vacuum tubes.
Label the tubes.

4. Urine collection (puncture)

The bladder should be full and exit the pelvis.
Before puncture, the skin is cleansed with a disinfectant solution.
The bladder is punctured with a needle along the midline of the abdomen, upward from the symphysis.
Transfer urine into test tubes using a syringe.
To test for anaerobes, urine from a needle is immediately placed into a special culture system.
Label the tubes.

5. Collection of the first morning urine sample for molecular diagnostic testing

Urine for molecular diagnostic testing is used only in men.
Before taking material for examination, you should not wash the external genitalia.
For analysis, you need to take the first 3-5 ml of urine from the first morning portion.
Collect urine in a sterile urine collection container.
Label the container.

6. Collecting urine during prostate massage

First of all, 10 ml of the middle part of urine is collected in the 1st container.
A massage of the prostate gland is performed per rectum.
Then 10 ml of urine is collected in another container
Containers must be labeled.

7. Collection of daily (24 hour) urine (tests for catecholamines, etc.)

Daily urine, that is, urine collected over 24 hours, is collected in a clean 2-3 liter graduated vessel with a tight-fitting lid. Urine collection containers can be obtained from the laboratory.
The patient does not need special instructions.
In the morning, on the first day of urine collection, you should empty your bladder into the toilet, while recording the time of urination (that is, the time when urine collection begins) in the direction.
NB! To determine catecholamines, calcium, magnesium and phosphate after the first urination, concentrated acid HCl is added to the urine container as a preservative. (HCl has a strong corrosive effect!). For the determination of catecholamines (metanephrine and normetanephrine) - 10 ml; Ca, Mg and P – 25 mL
Hydrochloric acid (HCl) will be given to you along with the container in the laboratory.
All subsequent urine samples should be collected in a urine collection container. Not a single portion should remain unaccounted for, that is, drained somewhere other than the container for collecting daily urine.
Between urinations, the urine collection container should be kept in a dark and cool place.
When adding each subsequent portion, the urine must be mixed.
The last portion of urine is taken the next morning, exactly at the same time when collection was started, recording in the direction the time of completion of urine collection.
At the end of urine collection, measure its volume using an existing scale and record the resulting amount in the direction.
You can send all the urine in this container to the laboratory, or you can send only 50 ml (after mixing the entire amount of urine well).
Label the container.

Where to store urine before donation

The ideal option is to submit immediately. It is allowed to wait 1-2 hours. Transportation will take this time. Sometimes it is not possible to send urine right away. Some types of examination require the collection of daily secretions. It is important to properly preserve any material.

Storage requirements:

temperature +2-4 degrees;
protection from light;

sealed, sterile container;
strict adherence to deadlines.

It is not advisable to leave it at room conditions. At parameters above 15 degrees, sediment forms and chemical parameters change.

Urine, feces for testing, sputum, saliva, and other biomaterials cannot be frozen or filled with disinfectants or potassium permanganate.

Can urine be stored in the refrigerator?

We’ve come to the most frequently asked question: is it possible to store urine in the refrigerator before taking the test? From a temperature point of view, this is an ideal place. Just don’t put it on the door with saline solution, iodine, or other medications. Each time you open it, the temperature rises significantly.

The ideal place is the central shelf. We recommend removing the products. It is advisable to wrap the jar in a dark bag. The contents will be stored without light and will not disturb household members.

How long can urine be stored for analysis?

The duration is influenced by the purpose of the study. For some tests, biological fluid does not need to be stored at all. Only time is given for transportation. Sometimes a person comes to urinate at a laboratory reception point. He is given sterile containers and a toilet.

In a refrigerator

The material is stored in the refrigerator for the maximum period. Subject to immediate placement. Let's look at the time for the most popular methods.

Features of collection and storage of analyzes

How long does urine last in the refrigerator?

up to 120 minutes general analysis. Only morning urine is used and immediately sent for diagnosis. The goal is to examine the chemical sediment and identify inflammatory processes;
A three-state sample is stored for up to 24 hours. All urine from the first morning urination is collected and distributed into three containers. The purpose of the analysis is a thorough study of the course of inflammatory processes in the case of cystitis, pyelonephritis, and other genitourinary diseases;

Material for studying according to Nechiporenko is stored for up to 60 minutes. Immediate transport required. The goal is to identify diseases of the urinary system. Usually this is pyelonephritis, cystitis;
Urine is stored for up to 24 hours for analysis according to Kakovsky-Addis. All secretions are collected in a large container, mixed, and up to 100 ml of total liquid is taken. The goal is to identify diseases of the genitourinary system.

It is advisable to discuss the specifics of urine collection and the amount of material with your doctor.

At room temperature

How long urine can be stored for analysis at room temperature depends on the purpose of the diagnosis. Most laboratory tests require exclusively fresh urine. The maximum period is 120 minutes.

What shortens it:

temperature above +20 degrees;

using an unsealed container;

lack of sterility.

Dirty dishes and improper collection distort diagnostic results. Before taking the tests, preparation is required. You should wash your genitals with a non-fragrant hygiene product. Children should not apply cream or use powder. It is prohibited to collect urine from women during menstruation.

If urine had to be stored longer than expected or the temperature was not maintained, we recommend rescheduling the test to the next day. Most laboratories accept biomaterial with an expired delivery date.

The question of how long urine can be stored for analysis is extremely important, since the quality of the material directly affects the research results.

In addition to the conditions and expiration date, it is also necessary to comply with the collection rules. All this will help to achieve the most reliable biomaterial data.

How to properly prepare for a general urine test

Different laboratories may use different research methods and units of measurement. In order for the assessment of the examination results to be correct and the results to be acceptable, it is advisable to conduct research in the same laboratory at the same time.

Urine donation should be done after thorough toileting of the external genitalia so that the number of red and white blood cells is not artificially increased. The urine test should be taken in a disposable container. It is advisable to use a medium portion of urine. During menstruation, women are advised to refrain from taking a urine test.

General urine analysis

For a general urine test, it is preferable to use “morning” urine, which collects in the bladder during the night.

Urine should be collected after a thorough toilet of the external genital organs (failure to comply with this rule may lead to the detection of an increased number of red and white blood cells in the urine, which will complicate the correct diagnosis) into dry, clean dishes, well washed from cleaning agents and disinfectants - disposable ones must be used containers.

All urine can be collected for analysis, however, it may contain elements of inflammation of the urethra and external genitalia. Therefore, as a rule, the first portion of urine is not used. The second, middle, portion of urine is collected in a clean container, without touching the body with the bottle. The container with urine is tightly closed with a lid.

Urine collected for general analysis can be stored for no more than 1.5 - 2 hours (necessarily in the cold!). Long-term storage of urine at room temperature leads to changes in physical properties, cell destruction and bacterial growth.

You should not collect urine during menstruation.

It is not recommended to eat vegetables and fruits that can change the color of urine (beets, carrots, etc.)

It is not recommended to take diuretics.

On the eve of the test, you should exclude alcohol, marinades, smoked foods, and honey.

Collection of 24-hour urine for biochemical analysis

Daily urine is collected within 24 hours with normal drinking regimen.

In the morning at 6 - 8 o'clock the bladder is emptied (this portion of urine is poured out), and then during the day all the urine is collected in a clean wide-necked vessel with a tight-fitting lid, with a capacity of at least 2 liters.

In this case, the container with urine must be kept at all times in a cool place (optimally in the refrigerator on the bottom shelf - at 4 - 8 C), preventing it from freezing.

The last portion is taken exactly at the same time when the collection began the day before (the start and end times of the collection are noted).

If not all urine is delivered to the laboratory, then the amount of daily urine is measured with a measuring cylinder, part is poured into a clean vessel in which it is delivered to the laboratory, and the volume of daily urine must be indicated.

Before submitting urine for analysis, it is undesirable to use medicinal substances, since some of them (in particular, ascorbic acid, which is part of complex vitamin preparations) affect the results of biochemical tests of urine; diuretics can significantly distort test results.

On the day of urine collection, physical activity should be avoided (they can cause the appearance of protein in the urine); urine collection should not be carried out in the presence of an infectious disease with elevated body temperature, or a significant increase in blood pressure.

Zimnitsky test

Excessive fluid intake is not allowed; avoid diuretics on the day of the study. The test is carried out with the patient's usual drinking regimen and nutrition. Eating spicy and salty foods is not recommended.

Urine is collected throughout the day, every 3 hours (8 servings), including at night.

At 6 am the patient empties the bladder, this portion is poured out.

Then, exactly every 3 hours, all the urine that has accumulated during this time is collected in a separate, clean, dry container. It is necessary to collect urine at 9, 12, 15, 18, 21, 24, 3 and 6 hours. Each container is placed in a cool, dark place.

Each container must indicate the portion number, the volume of urine excreted and the time of collection.

Urine collection ends at 6 am the next day.

Urine analysis according to Nichiporenko

Immediately after sleep (on an empty stomach), collect an average portion of morning urine with free urination. When urinating, men must completely retract the skin fold and release the external opening of the urethra. Women should spread their labia.

Urine collection is carried out using the “three-glass” sample method: the patient begins to urinate in the first glass, continues in the second, and finishes in the third. The predominant volume should be the second portion, which is collected in a disposable container (without touching the body).

The collected medium portion of urine (20-25 ml) is delivered to the laboratory no later than 2 hours after it was collected.

Bacteriological culture of urine

Urine for research is collected in the morning, or after 2-3 hours of abstaining from urination, after toileting the external genitalia, into a sterile disposable container.

Traditionally, the middle portion is collected (for this, the first amount of urine excreted (15-20 milliliters) is passed, and the middle portion of morning urine is placed in a sterile container).

The analysis is collected before a course of antibiotics or 2-3 weeks after it.

Urinalysis for 17-KS

It is necessary to collect urine for 24 hours with normal drinking regimen.

48 hours before urine collection and on the day of collection, you should not eat beets and carrots, exclude bananas, pineapples, tomatoes, eggs, chocolate, cheese, as well as foods containing vanillin. It is advisable to exclude spicy and salty foods. It is necessary to limit as much as possible the intake of products containing caffeine and other stimulants (tea, coffee, cocoa, Coca-Cola). If possible, avoid taking medications 1-2 days before the study, except those used for health reasons.

In the morning at 6-8 o'clock you need to empty your bladder (this portion of urine is poured out), and then during the day you must collect all the urine in a clean wide-necked vessel (jar) with a capacity of at least 2 liters. The last portion is taken exactly at the same time when urine collection began the day before. The amount of daily urine is measured using a measuring cup, a small amount is poured into a jar (approximately 100 ml) and delivered to the laboratory.

Be sure to indicate the volume of daily urine.

When collecting urine during the day, it is recommended to store it in a cool place.

Take tests constantly in the same laboratory - and your doctor will approximately know your personal normal values and any deviation from the norm will be immediately noticed by him.
Home visit Addresses of medical offices Frequently asked questions Personal data processing policy

Properties of urine

Urine contains a large amount of organic and inorganic substances that can enter into a chemical reaction when released into the external environment, thereby changing the composition of the liquid .

In a short time, sediment forms in the urine, and this significantly reduces the quality of the material. Then bacteria appear in it, which also change the composition with the products of their vital activity.

In addition, a large amount of ammonia , which also affects the analysis results. Organic compounds quickly break down and begin to interact with each other, forming byproducts.

However, all these processes can be slowed down when it is not possible to quickly deliver urine to the laboratory.

Where and how to properly store until morning?

Urine collected within 2 hours . The more this indicator is exceeded, the lower the quality and accuracy of urine testing.

After 24 hours, the material is completely unsuitable for analysis, regardless of where and how it was stored.

Let's look at the recommended storage times for various types of urine tests:

General analysis - given in the morning. It is not advisable to store or refrigerate. The material must be delivered within an hour.
Three-glass sample - taken in the morning. Can be stored for up to an hour at room temperature.

Read our article on how to collect a three-glass urine sample.

The best way to increase shelf life may be in the refrigerator. However, the temperature should not be lower than +4°C , since freezing will completely damage the material.

Preservatives will help to store daily urine :

boric acid – 3 granules for every 100 ml;
thymol – 2 granules for every 100 ml;
acetic acid (glacial) – 5 ml for any volume;
toluene – 2 ml for any volume.

Under no circumstances should urine be frozen. It completely loses its properties and structure and is not suitable for analysis.

Urinalysis: Frequently Asked Questions

A general urine test is a very informative and therefore common laboratory test. But the information may not be completely reliable if you ignore some rules. Tatyana Nikolaevna Yankovaya, candidate of medical sciences, gastroenterologist at the Expert Clinic Smolensk, told us how to donate urine for analysis and what indicators are important to pay attention to.

— Tatyana Nikolaevna, are clinical and general urine tests the same thing or is there a difference?

— These concepts are synonymous and denote a standard laboratory test.

— What indicators are paid attention to during a general (clinical) urine analysis? What are the characteristics of urine from a healthy person?

— First, color, transparency and the presence of sediment are assessed. In a healthy person, the urine is yellow, transparent, and there is no sediment. Acidity is another important indicator. The normal pH value is 4. Specific gravity is from 1012 to 1022 g/ml. Protein is normally absent, but trace amounts can be detected. Also, the urine should not contain glucose, bilirubin, ketone bodies and, of course, bacteria and fungi. There should be no more than 3 leukocytes in the field of view.

— What diseases can be diagnosed using a general urine test?

— Urinalysis is important for assessing the condition of the body as a whole. But for diseases of the urinary system it is of particular importance. The presence of bacteria in the urine indicates an infection in the kidneys or bladder. In case of urolithiasis, the type of stones can be determined by microscopic examination of the solid sediment.

Read materials on the topic:

A delicate problem: what you need to know about cystitis? Pyelonephritis: what is this disease and how to treat it? “This is not the time to collect stones”: what is urolithiasis?

Blood diseases also greatly change the results of a urine test. For example, during hemolytic processes, bilirubin appears in the urine.

The presence of protein in the urine usually indicates inflammatory processes. Protein also appears in the urine during injuries, intoxication, and diabetes. A sign of diabetes mellitus is the appearance of glucose and ketone bodies.

Read more about diabetes in our article

— How much urine is needed for analysis?

- 100-150 milliliters.

— Do I need a special container or is any container suitable for collecting urine?

— Due to an incorrectly selected container, the results may be distorted. If the container was not sterile, the test will show the presence of bacteria. Also, protein compounds may remain on the walls of the container, therefore, the protein test will be false positive.

Volume is also important. Therefore, it is better to give preference to special sterile containers.

— What other factors can affect the reliability of the result?

- There are many of them. Urine test results may change while taking medications. Nutrition before collecting material for analysis and external factors also influence. If a person sweats a lot due to the heat, the urine will be more concentrated and its density will increase. During intense physical activity, protein may appear in the urine of a healthy person.

The results are also distorted if the collection technique is violated.

— How to properly collect urine for analysis?

— When preparing for a urine test, it is advisable to follow a standard regimen and avoid strenuous physical exertion. A few days before the test, you need to avoid drinking alcohol and quit smoking. If you are taking any medications, tell your doctor in advance. He will decide whether it is possible to stop taking it for a while before the analysis. If not, the information will be taken into account when interpreting the results.

You need to collect urine in the morning, on an empty stomach, after a night's sleep. It is necessary to collect an average portion of urine in a sterile container. The material for analysis cannot be stored; it must be delivered to the laboratory as quickly as possible (within an hour or an hour and a half).

Perhaps you are interested in information about other tests? Read our articles in the section on laboratory diagnostics. You can sign up and take a urine test and some others here. ATTENTION: the service is not available in all cities

Interviewed by Daria Ushkova

The editors recommend:

What does a urine test according to Nechiporenko show? We detect kidney stones. CT, ultrasound or MRI - what to choose? Spring exacerbation: prevent and neutralize. Kidney and bladder diseases Does your lower back hurt? Don't panic! Laser on stones! How can lithotripsy help with urolithiasis?

For reference:

Yankovaya Tatyana Nikolaevna

She graduated from the medical faculty of the Smolensk State Medical Institute in 1993.

In 2002 she defended her Ph.D. thesis.

General practitioner, gastroenterologist at the Expert Clinic, Smolensk. Receives at the address: 8 March Street, 20.

How to collect urine from a baby?

To collect urine from an infant, use a special urine collector , which is sold in pharmacies. It consists of a sterile polyethylene container with a hole and Velcro for fixation.

It is forbidden to collect children's urine from a chamber pot or other similar place, since in any case there are bacteria and foreign substances that will distort the results of the examination. The container must be sterile. The minimum volume of urine should be 100 ml , and this amount can only be collected using a urine collector.

If you don't have a urine collection bag, you can use sterile glass jars or plastic bags.

Store baby urine in the refrigerator at a temperature of +4°C to +6°C for a maximum of 2 hours.

How to collect daily analysis?

The first urination in the morning is not used for analysis, but the exact time must be recorded. All subsequent times you should urinate in a special container, which is tightly closed and placed in the refrigerator. The last time they urinate is exactly 24 hours after the first urination.

The total amount of liquid is then measured. The container is thoroughly shaken several times, and 100-150 ml is taken . It is important to indicate not only the total volume of urine, but also the amount of liquid drunk per day. Next, you need to quickly deliver the material to the laboratory.

The container for daily analysis must be sterile with a tight-fitting lid. Store the analysis in the refrigerator at a temperature not lower than +4°C and not higher than +8°C.

Preparation requirements for taking a urine test

It is recommended to test the morning urine sample.

Collect the morning urine sample in a clean, dry glass or plastic container with a lid (it is better to use special plastic containers with screw caps) and deliver it to the laboratory along with the directions (no later than 1.5-2 hours after urine collection).

For most urine tests, there are general requirements:

exclude alcohol from the diet for 24 hours before the test;
exclude from the diet spicy, salty foods, foods that change the color of urine (for example, beets, carrots) for 12 hours before the test;
Avoid taking diuretics for 48 hours before urine collection (in consultation with your doctor);
exclude physical and emotional stress during the collection of daily urine (during the day);
Before taking the test, perform a thorough toileting of the external genitalia;
For women, the study is recommended to be carried out before menstruation or 2 days after its end.

General urine analysis:

The entire morning portion is collected, from which the required amount is poured into a clean, dry glass or plastic container.

Urinalysis according to Nechiporenko:

The average portion of urine is collected immediately into a clean, dry glass or plastic container.

Urine test for MAU (microalbuminuria):

the entire morning portion is collected, from which the required amount is poured into a clean, dry glass or plastic container,

Urine is collected per day (24 hours) and the required amount is poured from the total amount of urine into a clean, dry glass or plastic container.

Bacteriological culture of urine:

The average portion of urine is collected immediately into a sterile plastic container. Urine collection must be carried out before the start of drug treatment and no earlier than 5 days after the course of treatment.

Urine analysis according to Zimnitsky:

8 portions of urine are collected during the day (every 3 hours) in a clean, dry glass or plastic container.

Rules for collecting daily urine

(extracted for 24 hours from the time of the first urination of the current day to the same hour the next day).
In the morning, for example, at 7.00 - the first portion of urine remaining in the bladder from the night is not collected, the patient urinates in the toilet. Subsequent portions of urine, regardless of time, are collected in one clean container with a volume of at least 3.0 liters (stored in the refrigerator). The last portion of urine is collected in the same container at 7.00 the next day.
The volume of urine collected per day is carefully (with an accuracy of 1 ml) measured and mixed, then a portion of about 100-150 ml of urine is poured into a clean, dry glass or plastic container for testing.
This portion of daily urine is immediately delivered to the CDL.
On the referral form, the patient must write the measured daily urine volume (diuresis) in milliliters, for example: “Urine volume per day is 1200 ml.”

Rules for collecting biological material for the Rehberg sample

Option 1 – from a portion of urine collected within an hour

In the morning, empty your bladder completely (throw away this portion of urine).
On an empty stomach, drink 400-500 ml of water or weak tea (without sugar). Mark the exact time.
Exactly after 1 hour, collect all the urine in a clean, dry glass or plastic container and deliver it to the laboratory.
In the middle of this hour (after half an hour), donate blood strictly on an empty stomach from a vein.

2nd option – from a portion of urine collected during the day

Urine per day is collected (excreted in 24 hours from the time of the first urination of the current day to the same hour the next day).
In the morning, for example, at 7.00 - the first portion of urine remaining in the bladder from the night is not collected, the patient urinates in the toilet. Subsequent portions of urine, regardless of time, are collected in one container with a volume of at least 3.0 liters (stored in the refrigerator). The last portion of urine collected in a container at 7.00 the next day.
The volume of urine collected per day is carefully (with an accuracy of 1 ml) measured and mixed, then a portion of about 100-150 ml of urine is poured into a clean, dry glass or plastic container for the Rehberg test.
This portion of daily urine is immediately delivered to the laboratory, and immediately in the treatment room it is necessary to take a blood test from a vein.
On the referral form, the patient must write the measured daily urine volume (diuresis) in milliliters, for example: “Diuresis 1200 ml.”

Urine analysis according to Zimnitsky

Collect urine for 24 hours at the usual drinking rate (1.5-2 liters per day), taking into account the amount of liquid drunk per day.
At 6 o'clock in the morning, empty your bladder (pour out this portion of urine).
Every 3 hours during the day, collect urine into separate, clean, dry glass or plastic containers, which indicate the time of collection and the portion number.

Total 8 servings:

1 serving - from 6-00 to 9-00 am, 2 servings - from 9-00 to 12-00,
3 serving - from 12-00 to 15-00, 4 serving - from 15-00 to 18-00,
5 portion - from 18-00 to 21-00, 6 portion - from 21-00 to 24-00,
7th portion - from 24-00 to 3-00, 8th portion - from 3-00 to 6-00.

Deliver the entire collected amount of urine in 8 containers to the laboratory.

General recommendations

For the most accurate and reliable results, you must follow the following recommendations:

a day before urine collection, give up alcohol;
You should not eat large quantities of carrots, blueberries, tomatoes, beets (they color the urine);
the day before the test, stop using any medications;
The use of diuretics is completely prohibited;
It is recommended to carry out hygiene measures immediately before collection;
The container for analysis must be crystal clear and have a tight-fitting lid.

If you follow all the recommendations, the test results will be as accurate as possible .

Find out how to properly store samples from the video:

How to properly prepare before taking the test
How long can urine be stored for analysis?
Where and how to properly store urine until the morning?
Is it possible to store urine for longer than the specified time?
Collection and storage of urine in children

The quality of the collected material for laboratory research determines the reliability of the results obtained. This also applies to urine tests. It is very important to collect it correctly, store it under certain conditions and adhere to the permissible shelf life. Only in this case will the analysis show the real picture of the disease, and the treatment will give results.

Preparing to collect urine for analysis

To get good material for analysis, you should start preparing a day before collecting urine -

refrain from eating spicy, salty and spicy foods;
do not eat foods that change the color of urine - beets, carrots and others;
do not drink alcohol, coffee and mineral water;
exclude sweet foods;
It is advisable not to take vitamins, antibiotics, antipyretics, or diuretics (you should consult your doctor about this);
stick to the usual amount of fluid you drink;
exclude heavy physical activity, visiting the sauna, baths.

It is also worth preparing a container for collecting urine in advance. The best option is a plastic container with a volume of 100-200 ml, sold in a pharmacy. But it can also be a glass jar with a tight lid that needs to be sterilized. In the morning, the jar should be dry without any traces of cleaning agents or disinfectants. It is better not to use plastic boxes - some types of plastic can release foreign impurities.

Since hygiene procedures will need to be carried out in the morning before urine collection, you should prepare in advance

means for washing – a weak solution of laundry soap (not toilet soap, not shower gel),
solution of furatsilin or potassium permanganate,
towel.

How to properly prepare before taking the test

Many people do not know how, where and whether it is even possible to store urine before taking an analysis. There are certain recommendations, following which will help you collect urine correctly and get the correct research result.

One day before the due date you cannot:

drinking alcohol;
eat apples and carrots;
take medications that may affect the composition of urine;
use diuretics.

Before taking tests, you need to do hygiene procedures. Only then should you begin collecting material.

If it is necessary to undergo a general analysis, then the liquid is taken from the “middle stream”, i.e. start and finish urinating in the toilet, and the urine from the middle of urination is collected in a special container.

How long can urine be stored for analysis?

Doctors and laboratory technicians insist that urine cannot be stored for more than 2 hours before it is sent to the laboratory. During this time, it changes its physical and chemical parameters, the sediment changes, and various microorganisms develop in the urine. They are the reason that standing urine changes its pH, which is often recorded by laboratory assistants conducting research. And due to the destruction of yellow pigments in light during storage, this indicator will be distorted.

Where and how to properly store urine until the morning?

If no more than 2 hours have passed from the moment of collection to delivery to the laboratory, then the errors in the indicators will not be significant. The longer the biomaterial is stored, the more the accuracy of the data obtained suffers.

Important! If urine has stood for a day, then regardless of the conditions in which it was kept, it is in no way suitable for analysis.

Depending on the type of analysis, urine can be stored:

24-hour urine is so called because it is collected over a 24-hour period. In this case, storage is permissible during this entire time in the refrigerator, but not in the freezer.
If a general analysis is prescribed, the fluid is collected in the morning and immediately delivered to the laboratory. It cannot be stored for more than 1 hour.
For a three-glass sample, urine can be kept for no more than 1 hour at room conditions. The collection takes place in the morning.
The Zimnitsky analysis involves collecting urine per day, 8 times in separate containers. They are kept in the refrigerator for no more than a day. This type of cooling is acceptable. The addition of preservatives is also permitted.
For the range of studies on Nichiporeno, urine is not cooled at all, this is prohibited. After morning urination, she should be delivered to the laboratory no later than an hour later.

A refrigerator will help extend the shelf life of urine for analysis, but provided that the material is not cooled below +4 degrees. You cannot freeze the liquid, this will completely change it.

Preparation and rules for collecting stool and urine analysis

To check the functioning of the gastrointestinal tract and urinary system, the doctor sends us to examine stool and urine tests. There are different options for taking these tests, depending on what the doctor wants to know. Therefore, do not rush to immediately run to the laboratory if you complain of frequent urination, lower back pain or abdominal discomfort with a jar of urine or feces, but contact a specialist who will recommend the right type of study and notify you on the spot about preliminary preparations .

But if you still go to the laboratory first, read this information - it will help you not make mistakes when preparing for the study. A coprogram is a study of stool to diagnose diseases of the digestive system. In order for the analysis to be correct, you need to adhere to certain rules when preparing for it and during the fence itself. If stool is collected with errors, the analysis may reveal undigested food, fats, starch, and the doctor may make an invalid diagnosis. That's why:

For analysis, bring “fresh” feces without foreign substances (water, urine), collected in a clean container with a lid (for example, a container that can be purchased at almost any pharmacy).
3 days before the study, stop taking medications that can change the structure and color of stool (laxatives, enzyme preparations, iron supplements, sorbents, rectal suppositories, oils, enemas).
Stick to a diet for 4-5 days (do not eat foods that contribute to gas formation, constipation or loose stools).
You cannot take a stool test after an X-ray examination of the intestines.
do not take the test during menstruation, as blood may get into the test material (and then, unfortunately, the doctor may suspect internal bleeding, rectal fissures, or more serious problems). If, however, the analysis cannot be postponed for several days, perform a thorough toilet of the genital organs, install a tampon, wash the external genitalia again and begin collecting the analysis.
Deliver the sample taken within 1-2 hours, at room temperature, do not freeze, do not overheat.

To study the intestinal microflora, feces are examined for dysbiosis

The collection rules correspond to the coprogram, but have several features. A stool test for dysbacteriosis is taken before starting antibiotics.

The analysis must be collected in a sterile container (vessel). To do this, wash the container with a disinfectant solution, rinse with boiling water, and dry.
put part of the stool (up to 1/3 of the volume) into a clean disposable container with a lid.
deliver to the laboratory no later than 3 hours; do not freeze.

To identify helminths (worms) in the body, feces are prescribed for helminth eggs.

The preparation is the same. The only difference is that for testing, feces must be taken from several different places. Fill the container halfway, close, and deliver to the laboratory.

Urine is a biological fluid with which metabolic products are removed from the body. Its analysis is very important in the diagnosis of many diseases of the urinary and excretory system.

Failure to comply with the rules for taking a urine test can lead to an incorrect diagnosis. So:

We collect morning urine on an empty stomach. We flush the first 1-2 seconds into the toilet, then 50-70 ml. in a clean, sealable container (can be purchased at almost any pharmacy or laboratory).
deliver no later than 1.5-2 hours to the laboratory.
on the eve of taking a urine test, do not consume foods and medications that can distort the color and content of urine (beets, carrots, citrus fruits, blueberries, vitamins, diuretics, mineral water...)
per day - alcohol is strictly contraindicated, avoid any stress (intense physical, emotional), as protein may appear in the urine.
The container for analysis must be sterile; if necessary, rinse with boiling water and dry.
Do not take a urine test during menstruation, as blood cells may appear in the urine. But if, nevertheless, the study cannot be postponed for a while, perform a thorough toilet of the genital organs, install a tampon, wash again, and then collect the analysis. When visiting a doctor, inform about menstruation during the period of urine collection.
If cystoscopy (examination of the bladder) was performed, postpone the test for 5-7 days.

In case of changes in the general urine test, to identify or exclude kidney diseases, the doctor prescribes a urine test according to Nechiporenko .

The preparation rules are the same, except that only the “middle portion” is used for analysis, that is: the first portion of urine into the toilet, then 20-30 ml. - into the container, and finish urinating into the toilet too.

To determine the concentration of urine, the doctor may recommend a Zimnitsky test (24-hour urine)

During the day, 8 portions of urine are collected.
For this you will need 8 containers with a volume of 1.5-2 liters. each. In advance, glue pieces of paper with the specified period of time onto all containers (containers, jars).
During the day - normal diet and drinking
Do not take diuretics
At 6 am the patient empties into the toilet, the first container collects urine from 9.00 to 12.00, the second from 12.00 to 15.00, and so on..., from 3.00 to 6.00 am - in the eighth. At night, set an alarm for every 3 hours so you can urinate, and finish collecting at 6:00 am the next day.
Store all containers in the refrigerator and deliver to the laboratory in the morning.
If there was no urine in a three-hour period, the container is delivered empty.

A daily urine test is prescribed by a doctor to determine the amount of glucose and other substances in the urine.

Preparation is the same as for general analysis.

But for this you will need a large, clean container (perhaps a 3-4 liter jar with a lid) and, starting, say, at 7.00 in the morning, place all portions of urine, except the first, in this container (you can urinate in a smaller container and drain it) until 7:00 am the next day. All this time, the container is stored in the refrigerator at a temperature of 4-8 degrees (but do not freeze), the next morning, shake all the contents thoroughly, pour out 100-120 ml. and deliver to the laboratory, indicating the total volume of urine per day.

The article was prepared by ACMD-MEDOX specialists

Is it possible to store urine for longer than the specified time?

Freshly collected urine is the best option for analysis, but it is not always possible to correctly collect and store it. Tests are often prescribed for children, infants, and patients who have serious problems with urination.

The work schedule also makes its own temporary adjustments. In these cases, it is worth remembering that the container with urine can be kept in the refrigerator for a certain time. There it should only cool down a little, but not start to freeze. The optimal temperature to preserve the properties of the material is +4 degrees. Such conditions in the refrigerator are created on the doors or on the shelf at the very bottom of the chamber.

Important! If the urine has been stored in the refrigerator since the evening, this point should be indicated to the laboratory assistant.

In some cases, to determine the full picture of the disease, patients need to collect all their urine per day. In this case, there is no way to do without storing it for more than 2 hours. Special preservatives will help preserve the properties of urine.

The following substances can be added:

Acetic acid (glacial). No more than 5 ml of liquid for the entire volume of urine collected per day.
Toluene. Use no more than 2 ml for the entire container. Such a substance can lead to sediment after several hours.
Boric acid. Add in the following doses: for every 100 ml, 3 granules.
Thymol. Use 2 crystals per 100 ml volume of urine.

Is it possible to store tests and how?

The period of urine retention depends on the temperature conditions, as well as the type of study. For some laboratory testing, you will need “overripe” urine. Nevertheless, biomaterials are still placed in the refrigerator for “aging”.

Urine does not last long. It is a perishable human product. The final result depends on how fresh the material was. It is better to collect biomaterial immediately before delivery, because it contains nitrogenous products of the breakdown of protein and salts. Due to the chloride, phosphate and sulfate organic components, urine breaks down into many components that are smaller in volume. These components react to each other. The substances included in the composition, as well as its qualitative characteristics, will change. 120 minutes after urine is collected, it becomes practically unsuitable for analysis in the laboratory.

The material for analysis must be collected in a special container and delivered to the laboratory no later than 2 hours after collection.

For a reliable conclusion, children’s urine and infant urine cannot be stored even in a refrigeration unit. It is important to place it in a urine collection bag and send it for testing immediately - ammonia is formed in it. Therefore, it is impossible to take urine tests later than the due date - the presence of ammonia in urine will be taken as a deviation from the norm, that is, a disease will be suspected. At room temperatures, it will be possible to keep the urine of an adult and a child normal if it stands for no more than 2 hours from the period when the liquid was collected. If stored refrigerated, this will extend the validity period by 4 hours from the time of fluid collection.

How long can a urine test be stored?

General urine analysis

The shelf life of biomaterial for general analysis varies from 1 to 24 hours. It all depends on where the biomaterials were stored. If the liquid is kept in the refrigeration unit all the time, then the maximum amount of time it can stand is 1 day. Then it becomes unusable. But laboratory assistants advise not to store the material for delivery, but to hand it in on the same day.

According to Nechiporenko

The analysis is prescribed to diagnose infections and inflammatory processes of the urinary tract.
If a patient needs to donate urine to study a substance according to Nechiporenko, then it is important to collect biomaterials at home under sterile conditions, then immediately take them to the clinic. It is worth providing the substance to the laboratory assistant very quickly. Cold storage is possible for no longer than one and a half to two hours from the moment the biomaterial is collected. Thus, the liquid will remain suitable for research.

According to Zimnitsky

To collect material for this type of laboratory research it will take more time: from 6 a.m., 8 times a day. It is stored in portions. That's why you need to stock up on clean, special containers. They must be large in volume so that all the material that a person produces at a time fits into the jar. Jars must be numbered. The collected urine should be kept in a refrigeration unit (no longer than 1 day).

Daily urine

The substance, which settles for 24 hours, is needed for study according to Kakovsky-Addis. Patients keep the biomaterial cold all the time. The main rule for collecting daily analysis is to maintain the sterility of the container into which the biomaterial is collected. Urine for daily testing can be stored exclusively in glass containers. Plastic pharmacy containers are not suitable for this, because the material begins to interact with urine breakdown products. A daily analysis will not work if the substance has been standing for more than a day.

Three-glass sample

It is important to submit the collected urine using the three-glass sample method for analysis in a timely manner.
When taking a 3-glass sample, a person defecates in such a way that the excreted biomaterial is divided into three portions: the first part of the urine is placed in glass No. 1, the middle portion of urine is placed in glass No. 2, and the remainder is placed in glass No. 3, and all glasses are signed . The storage conditions for the urine that will be needed for the sample are similar to other studies of this type - no longer than 120 minutes.